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KMID : 0613820040140060975
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Journal of Life Science
2004 Volume.14 No. 6 p.975 ~ p.985
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The Effects of EGEE on the Morphometry in the Thickness and Histogenesis of Rat Cerebral Cortex During Developmental Phase
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Lee Eung-Hee
Jeong Kil-Nam
Jo Gi-Jin
Jo Un-Bock
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Abstract
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This study attempts to investigate the developmental alterations of rat cerebral cortex, and the effects of EGEE on the developmental cerebral cortex in the prenatal, postnatal and adults were examined by morphological methods and H-E staining was used for the histological changes. In the case of injection of EGEE, at 14 day of fetal phase, parietal cortex was thickest (95±12.7 μm) but, it was thinner than in the control group (102±14.0 μm) and, occipital cortex (57±10.5 μm) compared with other cortexes was the thinnest in fetal phase. In the suckling phase, each cortex grew thick quickly but, after weanning phase, the growth of the cortex slowed and the thickness of cortex was similar to that of cortex in the adult phase. At 105 day after birth, the parietal cortex was thickest (934±21.6 μm) but, decreased compared with control group (1113±19.0 μm). When EGEE was injected in intraperitoneal of rat, the number of neuroblasts per unit area was largest (200.7±11.4/10-2 mm) at the mantle layer of parietal cortex at 14 day of fetal phase but, decreased compared with control group (224.2±13.8/10-2 mm), and the size was largest (7.5±1.3 μm) at the ependymal cell layer of occipital cortex at 3 day after birth but, decreased compared with control group (9.0±1.2 μm). Simillar to control group, the number of granular cells and pyramidal cells were largest at the II and III layer of parietal cortex, but decreased during developmental phase. The size was largest at the IV and V layer of occipital cortex but it was decreased compared with control group. When EGEE was injected in intraperitoneal of rat, the cerebral cortex from fetal phase to 3 day after birth has differentiated into the 3 layers; ependymal, mantle and marginal layer, but empty cisternaes or vacoules in the cerebral cortexes and the condensed phases of neuroblasts were appeared. From 5 day after birth, it has differentiated into the 4 layers; molecular, external granular, mixed layer of internal granular, external and internal pyramidal cells and multiformal layer but, empty cisternaes or vacoules in the granular and pyramidal cell layers were appeared and the number per unit area of neuron was decreased. In the cerebral cortex of the weaning and adult phases, division of cell layers was not clear and empty cisternae was formed in the cortex with the cells in external granular and pyramidal cell layers, was magnified or condensed around blood vessels of neurons.
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KEYWORD
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EGEE, Developmental Cerebral cortex, Neuroblast, Granular cell, Pyramidal cell.
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